Microscope Cleaning and Maintenance (from Zeiss)
Herman Lab Protocols:
WORM PROTOCOLS
EMS Mutagenesis
Generating Males by Heat Shock
Genomic Prep
Microinjections
PCR from Worms
UV array integration
Worm Decontamination
Worm Freezing
Worm Lysis
MOLECULAR BIOLOGY PROTOCOLS
5-minute transformation
Agarose Gel Electrophoresis
Antibiotic Usage
Competent Cells
Ethanol Precipitation
Freezing bacterial stocks
Herman Lab Media Additive List
Ligations
PCR Using Phusion
Phenol-Chloroform Extraction
Restriction Enzymes
Standard PCR Conditions